At the 2020 European liver academic research conference, liver disease researchers from different countries around the world published a comparative study on the specificity of hepatitis B virus nucleocapsid protein and envelope protein in patients with chronic hepatitis B. the research background and objectives are as follows:
researchers introduced that we developed a method to directly observe hepatitis B virus specific B cells. There are differences in phenotype and function between core antigen and surface antigen-specific B cells in patients with chronic hepatitis B. HBcAg specific B cells are class 1 switch memory B cells that secrete antibodies. HBV specific B cells express cross and innate immune genes. There is a certain correlation between HBV specific B cell function spectrum and serological data of patients with chronic hepatitis B.
researchers believe that the understanding of antiviral humoral immune regulation is limited when hepatitis B virus is naturally infected. Recently, the use of difluorochromium combined with HBsAg has proved that the function of HBsAg specific B cells in patients with chronic hepatitis B infection is impaired. However, the characteristics of HBcAg specific B cells are still unclear. At EASL 2020, researchers have developed a method to visually select and characterize HBcAg specific B cells that are parallel to HBsAg specific B cells.
the research method is as follows: the synthetic fluorescein conjugated HBcAg reagent was used to detect HBcAg specific B cells in 36 patients with chronic hepatitis B in vitro. The frequency, phenotype, functional maturity and transcriptional profile of hepatitis B core antigen-specific B cells were studied by flow cytometry, in vitro maturation test and nanowire immunoassay.
the results showed that the frequency of HBcAg specific B cells in all patients with chronic hepatitis B who participated in this study was higher than that of HBsAg specific B cells. Different from HBsAg specific B cells, they could effectively mature into antibody secreting cells in vitro. Their phenotype and transcriptome showed that HBcAg specific B cells were preferentially IgG + memory B cells. However, despite the differences in phenotype and function of HBcAg and HBsAg specific B cells in the patients with chronic hepatitis B, they have different mRNA expression patterns from global memory B cells.
this mRNA expression pattern is mainly characterized by high expression of genes with cross expression and innate immune activity. Based on the above research data, the researchers came to the conclusion and published it at the 2020 European liver academic conference: in the process of chronic hepatitis B virus infection, the serological detection of hepatitis B surface antibody and hepatitis B core antibody is directly related to the number and function of their specific B cells. However, transcriptome analysis in HBsAg and HBcAg specific B cells showed that HBV specific B cells had other functions besides producing antibodies.
in short, people usually have certain medical knowledge. For example, to protect themselves from hepatitis B virus infection, the body needs to produce antibodies. The researchers believe that the antibody is produced by special cells in the autoimmune system called B cells. During the period of chronic hepatitis B virus infection, the antibody inside the virus can be detected, but there is no antibody against the virus envelope.
through the development of a method, we can directly observe the B cells targeting the two viral components in vitro, highlight their differences and similarities, and show how the two components of the same virus have different effects on the function of anti viral B cells. The above data and conclusions are published in EASL At the 2020 conference, considering that the current global understanding of antiviral humoral immune regulation is insufficient under the background of hepatitis B virus natural infection, and the characteristics of hepatitis B core antigen specific B cells are not clear, a comparative study on the specificity of viral nucleocapsid protein and envelope protein in patients with chronic hepatitis B was published at the 2020 European liver conference. 21 days after delivery, the pregnant woman had a new baby, but the doctor did not find it was “twins”